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Gene Cloning and Manipulation Second Edition Christopher Howe University of Cambridge
CAMBRIDGE UNIVERSITY PRESS
Cambridge, New York, Melbourne, Madrid, Cape Town, Singapore, São Paulo Cambridge University Press The Edinburgh Building, Cambridge CB2 8RU, UK Published in the United States of America by Cambridge University Press, New York www.cambridge.org Information on this title: www.cambridge.org/9780521817936 © C. J. Howe 2007 This publication is in copyright. Subject to statutory exception and to the provision of relevant collective licensing agreements, no reproduction of any part may take place without the written permission of Cambridge University Press. First published in print format 2007 eBook (EBL) ISBN-13 978-0-511-29499-0 ISBN-10 0-511-29499-9 eBook (EBL) ISBN-13 ISBN-10
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Contents Preface to first edition Preface to second edition
Chapter 1 The tools for the job
page vii ix
1
Chapter 2
Polymerase chain reaction
30
Chapter 3
Simple cloning
52
Chapter 4
Other vector systems for E. coli
74
Chapter 5
Making libraries
98
Chapter 6
Screening libraries
116
Chapter 7
Modification and mutagenesis
143
Chapter 8
Use of cloned DNA
162
Chapter 9
Using other organisms
182
Chapter 10 References Index