E-Book Content
Gene Cloning and Manipulation Second Edition Christopher Howe University of Cambridge CAMBRIDGE UNIVERSITY PRESS Cambridge, New York, Melbourne, Madrid, Cape Town, Singapore, São Paulo Cambridge University Press The Edinburgh Building, Cambridge CB2 8RU, UK Published in the United States of America by Cambridge University Press, New York www.cambridge.org Information on this title: www.cambridge.org/9780521817936 © C. J. Howe 2007 This publication is in copyright. Subject to statutory exception and to the provision of relevant collective licensing agreements, no reproduction of any part may take place without the written permission of Cambridge University Press. First published in print format 2007 eBook (EBL) ISBN-13 978-0-511-29499-0 ISBN-10 0-511-29499-9 eBook (EBL) ISBN-13 ISBN-10 hardback 978-0-521-81793-6 hardback 0-521-81793-5 ISBN-13 ISBN-10 paperback 978-0-521-52105-5 paperback 0-521-52105-X Cambridge University Press has no responsibility for the persistence or accuracy of urls for external or third-party internet websites referred to in this publication, and does not guarantee that any content on such websites is, or will remain, accurate or appropriate. Contents Preface to first edition Preface to second edition Chapter 1 The tools for the job page vii ix 1 Chapter 2 Polymerase chain reaction 30 Chapter 3 Simple cloning 52 Chapter 4 Other vector systems for E. coli 74 Chapter 5 Making libraries 98 Chapter 6 Screening libraries 116 Chapter 7 Modification and mutagenesis 143 Chapter 8 Use of cloned DNA 162 Chapter 9 Using other organisms 182 Chapter 10 References Index