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Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined.The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.
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Menashe Marcus ( F e b r u a r y 20, 1 9 3 8 - J a n u a r y 2, 1987)
This volume is dedicated to the memory of Menashe Marcus, a major contributor to the concept and substance of this book, who died on January 2, 1987 at the age of 48. Menashe was a scientific colleague, collaborator, and friend to many of the coauthors of this work. All who knew him were enriched by his kindness, generosity, wonderful sense of humor, and intellectual honesty. His professional life was spent at the Hebrew University in Jerusalem. He was dedicated to the advancement of biological research in Israel through his own work, his efforts to introduce precise scientific terminology into modem Hebrew, and through his many successful and devoted students. He maintained strong professional and personal ties with the scientific community in the United States, and did his postdoctoral work at the Massachusetts Institute of Technology, with sabbatical appointments at Columbia University College of Physicians and Surgeons, New York University School of Medicine, and the National Institutes of Health. His enthusiasm and vigorous support for the idea that xiii
xiv the seeds sown in phage and bacterial genetics would bear fruit in the study of mammalian cells in culture has been borne out by the exciting developments of recent years. Guided by this precept, he pioneered techn