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Current Topics in Membranes and Transport Volume 20 Molecular Approaches to Epithelial Transport
Advisory Board
M . P. Blaustein G . Blobel J . S. Cook P. A. Knauf Sir H . L. Kornberg
P. Lauger C. A . Pasternak W. D. Stein W. Stoeckenius K. J . Ullrich
Contributors
Ralph J . Kessler William P. Alles R . Kinne Daniel Biemesderfer Ingeborg Koeppen Chris Clausen G. Kottra Malcolm Cox Simon A . Lewis M. E. M . Da Cruz J . J . Lim Troy E. Dixon J . T. Lin Darrell D. Fanestil Alicia A. McDonough Bliss Forbush III George K. Ojakian E. Fromter Lawrence G. Palmer Michael Geheb Chun Sik Park David B. P. Goodman Heide Plesken Victoria Guckian Uzi Reiss Michael C. Gustin Michael J . Rindler Doris A . Herzlinger B . C. Rossier T. Hoshiko David D. Sabatini Ivan Emanuilov Ivanov Bertram Sacktor L. Kampmann James B . Wade Michael Kashgarian N. K. Wills
Current Topics in Membranes and Transport Edited by
Arnost Kleinzeller
Felix Bronner
Department of Physiology University of Pennsylvania School of Medicine Philadelphia, Pennsylvania
Department of Oral Biology University of Connecticut Health Center Farmington, Connecticut
VOLUME 20 Molecular Approaches to Epithelial Transport Guest Editors James B. Wade
Simon A. Lewis
Department of Physiology Yale University School of Medicine New Haven, Connecticut
Department of Physiology Yale University School of Medicine New Haven, Connecticut
Volume 20 is part of the series (p. xix) from the Yale Department of Physiology under the editorial supervision of:
Joseph F. Hoffman Department of Physiology Yale University School of Medicine New Haven, Connecticut
Gerhard Giebisch Department of Physiology Yale University School of Medicine New Haven, Connecticut
1984
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COPYRIGHT @ 1984, BY ACADEMIC PRESS,INC. ALL RIGHTS RESERVED. NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM O R BY ANY MEANS, ELECTRONIC OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT PERMISSION IN WRITING FROM THE PUBLISHER.
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The editors wish to dedicate this volume to Dr. J . J . Lim, author of Chapter 2 . The tragedy of his death (September 1, 1983) on Korean Air Lines flight 007 will be felt as a personal and professional loss to all of us.
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Contents Contributors, xiii Preface, xvii Yale Membrane Transport Processes Volumes, xix
PART I.
CHAPTER
FREQUENCY DOMAIN ANALYSIS OF ION TRANSPORT
1.
Fluctuation Analysis of Apical Sodium Transport T . HOSHIKO
I. 11. 111.
IV. V. VI.
Introduction, 3 Mechanics of Fluctuation Analysis, 4 Epithelial Sources of Fluctuations. 7 Fluctuations in Apical Sodium Conductance, 1 1 Model Predictions, 14 Conclusion, 23 References, 24
CHAPTER
2.
Impedance Analysis of Necturus Gallbladder Epithelium Using Extra- and lntracellular Microelectrodes J. J. LIM, G . KOTTRA, L. KAMPMANN, AND E. FROMTER
1.
Introduction, 27
11. Data Analysis Technique, 29 111. Equivalent-Circuit Models of Necturus Gallbladder Epithelium, 32
IV. V.
VI.
Transepithelial and Apparent Intracellular Transfer Funct