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Current Topics in Membranes and Transport Volume 20 Molecular Approaches to Epithelial Transport Advisory Board M . P. Blaustein G . Blobel J . S. Cook P. A. Knauf Sir H . L. Kornberg P. Lauger C. A . Pasternak W. D. Stein W. Stoeckenius K. J . Ullrich Contributors Ralph J . Kessler William P. Alles R . Kinne Daniel Biemesderfer Ingeborg Koeppen Chris Clausen G. Kottra Malcolm Cox Simon A . Lewis M. E. M . Da Cruz J . J . Lim Troy E. Dixon J . T. Lin Darrell D. Fanestil Alicia A. McDonough Bliss Forbush III George K. Ojakian E. Fromter Lawrence G. Palmer Michael Geheb Chun Sik Park David B. P. Goodman Heide Plesken Victoria Guckian Uzi Reiss Michael C. Gustin Michael J . Rindler Doris A . Herzlinger B . C. Rossier T. Hoshiko David D. Sabatini Ivan Emanuilov Ivanov Bertram Sacktor L. Kampmann James B . Wade Michael Kashgarian N. K. Wills Current Topics in Membranes and Transport Edited by Arnost Kleinzeller Felix Bronner Department of Physiology University of Pennsylvania School of Medicine Philadelphia, Pennsylvania Department of Oral Biology University of Connecticut Health Center Farmington, Connecticut VOLUME 20 Molecular Approaches to Epithelial Transport Guest Editors James B. Wade Simon A. Lewis Department of Physiology Yale University School of Medicine New Haven, Connecticut Department of Physiology Yale University School of Medicine New Haven, Connecticut Volume 20 is part of the series (p. xix) from the Yale Department of Physiology under the editorial supervision of: Joseph F. Hoffman Department of Physiology Yale University School of Medicine New Haven, Connecticut Gerhard Giebisch Department of Physiology Yale University School of Medicine New Haven, Connecticut 1984 @ ACADEMIC PRESS, INC. (Harcourr Brace Jovanovich, Publishers) Orlando San Diego San Francisco New York London Toronto Montreal Sydney Tokyo Slo Paul0 COPYRIGHT @ 1984, BY ACADEMIC PRESS,INC. ALL RIGHTS RESERVED. NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM O R BY ANY MEANS, ELECTRONIC OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT PERMISSION IN WRITING FROM THE PUBLISHER. ACADEMIC PRESS, INC. Orlando, Florida 32887 United Kingdom Edition published by ACADEMIC PRESS, INC. ( L O N D O N ) LTD. 24/28 Oval Road, London N W I 7DX LIBRARY OF CONGRESS CATALOG CARDNUMBER: 70- I I 709 I ISBN 0-12-153320-4 PRINTED IN THE UNITED STATES OF AMERICA 84 85 86 87 9 8 7 6 5 4 3 2 1 The editors wish to dedicate this volume to Dr. J . J . Lim, author of Chapter 2 . The tragedy of his death (September 1, 1983) on Korean Air Lines flight 007 will be felt as a personal and professional loss to all of us. This Page Intentionally Left Blank Contents Contributors, xiii Preface, xvii Yale Membrane Transport Processes Volumes, xix PART I. CHAPTER FREQUENCY DOMAIN ANALYSIS OF ION TRANSPORT 1. Fluctuation Analysis of Apical Sodium Transport T . HOSHIKO I. 11. 111. IV. V. VI. Introduction, 3 Mechanics of Fluctuation Analysis, 4 Epithelial Sources of Fluctuations. 7 Fluctuations in Apical Sodium Conductance, 1 1 Model Predictions, 14 Conclusion, 23 References, 24 CHAPTER 2. Impedance Analysis of Necturus Gallbladder Epithelium Using Extra- and lntracellular Microelectrodes J. J. LIM, G . KOTTRA, L. KAMPMANN, AND E. FROMTER 1. Introduction, 27 11. Data Analysis Technique, 29 111. Equivalent-Circuit Models of Necturus Gallbladder Epithelium, 32 IV. V. VI. Transepithelial and Apparent Intracellular Transfer Funct