E-Book Overview
Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process. Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps. What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.
E-Book Content
GOLD and SILVER STAINING:
Techniques in Molecular Morphology
Advances in Pathology, Microscopy & Molecular Morphology Series Editors Jiang Gu and Gerhard W. Hacker PUBLISHED TITLE Gold and Silver Staining: Techniques in Molecular Morphology Gerhard W. Hacker and Jiang Gu
Advances in Pathology, Microscopy & Molecular Morphology Series Editors Jiang Gu and Gerhard W. Hacker
GOLD and SILVER STAINING:
Techniques in Molecular Morphology
Edited by
GERHARD W. HACKER Forschungsinstitut fuer Grund- und Grenzfragen der Medizin und Biotechnologie St. Johanns Spital, Landeskliniken Salzburg Salzburg, Austria, EU
JIANG GU
Department of Biomedical Sciences University of South Alabama Mobile, Alabama
CRC PR E S S Boca Raton London New York Washington, D.C.
Library of Congress Cataloging-in-Publication Data Gold and silver staining : techniques in molecular morphology / Gerhard W. Hacker, Jiang Gu. p. cm. Includes bibliographical references and index. ISBN 0-8493-1392-9 (alk. paper) 1. Immunogold labeling 2. Silver staining (Microscopy) 3. Histochemistry. I. Hacker, Gerhard W. II. Gu, Jiang. QR187.I482 G65 2002 611′.018—dc21
2002017541 CIP
This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use. Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, microfilming, and recording, or by any information storage or retrieval system, without prior permission in writing from the pub