E-Book Overview
Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.
E-Book Content
METHODS IN MOLECULAR BIOLOGY ™
329
Embryonic Stem Cell Protocols Volume 1: Isolation and Characterization
SECOND EDITION Edited by
Kursad Turksen
Embryonic Stem Cell Protocols, Vol. 1 SECOND EDITION
M E T H O D S I N M O L E C U L A R B I O L O G Y™
John M. Walker, SERIES EDITOR 346. Dictyostelium discoideum Protocols, edited by 346 Ludwig Eichinger and Francisco Rivero-Crespo, 2006 345 345. Diagnostic Bacteriology Protocols, Second Edition, edited by Louise O'Connor, 2006 344 344. Agrobacterium Protocols, Second Edition: Volume 2, edited by Kan Wang, 2006 343. Agrobacterium Protocols, Second Edition: 343 Volume 1, edited by Kan Wang, 2006 342 342. MicroRNA Protocols, edited by Shao-Yao Ying, 2006 341. Cell–Cell Interactions: Methods and Protocols, edited by Sean P. Colgan, 2006 340. Protein Design: Methods and Applications, edited by Raphael Guerois and Manuela López de la Paz, 2006 339. Microchip Capillary Electrophoresis: Methods 339 and Protocols, edited by Charles Henry, 2006 338. Gene Mapping, Discovery, and Expression: 338 Methods and Protocols, edited by M. Bina, 2006 337. Ion Channels: Methods and Protocols, edited by 337 J. D. Stockand and Mark S. Shapiro, 2006 336. Clinical Applications of PCR: Second Edition, 336 edited by Y. M. Dennis Lo, Rossa W. K. Chiu, and K. C. Allen Chan, 2006 335 335. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols, edited by Vladimir V. Didenko, 2006 334. PRINS and In Situ PCR Protocols: Second 334 Edition, edited by Franck Pellestor, 2006 333. Transplantation Immunology: Methods and 333 Protocols, edited by Philip Hornick and Marlene Rose, 2006 332. Transmembrane Signaling Protocols: Second 332 Edition, edited by Hydar Ali and Haribabu Bodduluri, 2006 331. Human Embryonic Stem Cell Protocols, edited 331 by Kursad Turksen, 2006 330. Embryonic Stem Cell Protocols, Second Edition, 330 Vol. II: Differentiation Models, edited by Kursad Turksen, 2006 329. Nonhuman Embryonic Stem Cell Protocols, 329 Second Edition, Vol. I: Isolation and Characterization, edited by Kursad Turksen, 2006 328. New and Emerging Proteomic Techniques, 328 edited by Dobrin Nedelkov and Randall W. Nelson, 2006 327. Epidermal Growth Factor: Methods and Protocols, 327 edited by Tarun B. Patel and Paul J. Bertics, 2006 326. In Situ Hybridization Protocols, Third Edition, 326 edited by Ian A. Darb