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The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
E-Book Content
Preface The articles in this volume were assembled to enable the reader to design effective strategies for the expression of cloned genes and cDNAs. More than a compilation of papers describing the multitude of techniques now available for expressing cloned genes, this volume provides a manual that should prove useful for solving the majority of expression problems one is likely to encounter. The four major expression systems commonly available to most investigators are stressed: Escherichia coli, Bacillus subtilis, yeast, and mammalian cells. Each of these systems has its advantages and disadvantages, details of which are found in Chapter [ 1] and the strategic overviews for the four major sections of the volume. The papers in each of these sections provide many suggestions on how to proceed if initial expression levels are not sufficient. DAVID V. GOEDDEL
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Contributors to Volume 185 Article numbers are in parentheses following the names of contributors. A~liationslistedare current.
LARS ABRAHMSl~N(13), Department of BiD-
CHRISTINAY. CHEN (35, 37), Department of
molecular Chemistry, Genentech Inc., South San Francisco, California 94080 PAULINA BALBAS(3), Departamento de Biologia Molecular, Centro de Investigacion sobre Ingenieria Genbtica y Biotechnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico CLINTON E. BALLOU (36), Department of Biochemistry and Molecular Biology, University of California at Berkeley, Berkeley, California 94720 RAMA M. BELAGAJE (8), Division of Molecular and Cell Biology, Lilly Research Laboratories, Indianapolis, Indiana 46285 ANNE B