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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today - truly an essential publication for researchers in all fields of life sciences.Key Features* Phage display libraries* Repression fusion proteins* Polysome libraries* Peptide libraries* Nucleic acid libraries* Other small molecule libraries
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Preface Combinatorial chemistry is a field that did not exist five years ago but is so vibrant today, especially in medicinal chemistry, that almost every major pharmaceutical company has a group working in this area and many start-up companies have been formed with combinatorial chemistry as their raison d'etre. Like many other fast-breaking developments, this field had its main origins in work done in academic research laboratories, and many of the techniques were developed to solve specific problems in basic research. The common feature of all combinatorial approaches is the generation of a complex mixture of molecules coupled to screens or selections which can identify out of that mixture a single molecule with desired properties, e.g., as the ligand or inhibitor of an enzyme or as a macromolecule with novel or enhanced properties. At the start most combinatorial libraries were of biological molecules, mostly peptides or nucleic acids, but because these molecules only rarely exhibit good pharmacological properties, increasingly the libraries of interest to medicinal chemists are of small molecules with a range of pharmacologically attractive properties. Because of the rapid progress in this field, a follow-up to th