E-Book Overview
Ian Darby updates the highly successful premier edition with a full panoply of new and greatly augmented state-of-the-art techniques. This valuable new edition contains basic and advanced in situ hybridization techniques-many not covered in the first edition-and includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA or RNA probes to detect expression in cells or tissue sections. Prepared by leading investigators who have daily worked with the techniques, fine-tuning them for reliability, In Situ Hybridization Protocols, 2nd Edition, offers a completely updated and extended collection of proven and readily reproducible methods suitable for both the novice and experienced investigator. Its state-of-the-art protocols constitute the new standard methods resource in the field, one that will enable researchers successfully to enhance and improve their current experimental repertoire.
E-Book Content
Methods in Molecular Biology
TM
VOLUME 123
In Situ Hybridization Protocols Second Edition Edited by
Ian A. Darby
HUMANA PRESS
Human Partial Chromosome Paints
3
1 Preparation of Human Partial Chromosome Paints from Somatic Cell Hybrids Nicoletta Archidiacono, Rosalia Marzella, Cosma Spalluto, Margherita Pennacchia, Luigi Viggiano, and Mariano Rocchi 1. Introduction Whole chromosome painting libraries (WCPLs) have provided a very powerful tool to cytogeneticists. The technique allows the painting of specific chromosomes in metaphase spreads and in interphase nuclei (1–4). The usefulness of WCPLs is particularly evident in identifying the chromosomal origin of de novo unbalanced translocations and marker chromosomes, or,