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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.Key Features* Analysis of Gene Expression* Gene Expression Systems for Functional Analysis* Yeast Expression Systems* RNA-based Control of Recombinant Gene Expression* Small Molecule Control of Gene Expression* Viral Systems for Recombinant Gene Expression
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Preface The ability to express recombinant proteins in eukaryotic cells has greatly increased our ability to study protein function in a variety of settings. Initial work in this field focused on gaining an understanding of the cis-acting sequences necessary for m R N A expression, processing, and translation. As the field has matured, the more sophisticated task of finely regulating gene expression has taken center stage. Researchers want to be able to limit expression to particular tissues and to determine the timing and level of expression. In addition, exciting new technologies are being developed that use small R N A molecules to regulate expression of endogenous genes. In this volume of Methods in Enzymology, we have brought together a number of exciting new techniques for regulated gene expression in eukaryotic cells. We hope that this volume will prove to be a valuable resource for those who are looking for new methods to express recombinant proteins in eukaryotic systems. We realize that no single volume can be complete, and certainly there are methods for recombinant protein expression not represented here. However, w