Crispr: Methods And Protocols

Methods in Molecular Biology 1311 Magnus Lundgren Emmanuelle Charpentier Peter C. Fineran Editors CRISPR Methods and Protocols METHODS IN MOLECULAR BIOLOGY Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 CRISPR Methods and Protocols Edited by Magnus Lundgren Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden Emmanuelle Charpentier Helmholtz Centre for Infection Research, Department of Regulation in Infection Biology, Braunschweig, Germany; The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Department of Molecular Biology, Umeå University, Umeå, Sweden; Hannover Medical School, Hannover, Germany Peter C. Fineran Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand Editors Magnus Lundgren Department of Cell and Molecular Biology Uppsala University Uppsala, Sweden Peter C. Fineran Department of Microbiology and Immunology University of Otago Dunedin, New Zealand Emmanuelle Charpentier Helmholtz Centre for Infection Research Department of Regulation in Infection Biology Braunschweig, Germany The Laboratory for Molecular Infection Medicine Sweden (MIMS) Umeå Centre for Microbial Research (UCMR) Department of Molecular Biology Umeå University Umeå, Sweden Hannover Medical School Hannover, Germany ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2686-2 ISBN 978-1-4939-2687-9 (eBook) DOI 10.1007/978-1-4939-2687-9 Library of Congress Control Number: 2015937760 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for a