E-Book Overview
This text takes the reader on a guided tour through the philosophical and physical foundations of protein isolation. Aimed at a student readership, it will also be very useful to life science researchers faced with the task of isolating a protein for the first time. The logic of the overall approach to isolating a protein is explained and the physical principles of each separation method are made clear by the use of simple models and analogies, drawn from everyday experiences. The author's aim has been to deepen the readers' insight into protein isolation methods, so that they may tackle new problems and perhaps devise new approaches to old problems. Many of the methods described are drawn from the author's own research and are thus uniquely described here: examples are three-phase partitioning, non-linear electrophoresis, and a simple approach to buffer making.
E-Book Content
A GUIDE TO PROTEIN ISOLATION
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A GUIDE TO PROTEIN ISOLATION
by
Clive Dennison School of Molecular mid Cellular Biosciences, University of Natal,
Pietermaritzburg. South Africa
KLUWER ACADEMIC PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW
eBook ISBN: Print ISBN:
0-306-46868-9 0-792-35751-5
©2002 Kluwer Academic Publishers New York, Boston, Dordrecht, London, Moscow All rights reserved No part of this eBook may be reproduced or transmitted in any form or by any means, electronic, mechanical, recording, or otherwise, without written consent from the Publisher Created in the United States of America Visit Kluwer Online at: and Kluwer's eBookstore at:
http://www.kluweronline.com http://www.ebooks.kluweronline.com
Contents
Acknowledgements........................................................
ix
Preface.....................................