Rna-ligand Interactions, Part B

E-Book Overview

RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods.RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

E-Book Content

Preface A decade has passed since Methods in Enzymology addressed methods and techniques used in R N A processing. As has been evident since its inception, research in R N A processing progresses at a rapid pace. Its expansion into new areas of investigation has been phenomenal with novel discoveries being made in a variety of subspecialty areas. The subfield of RNA-ligand interactions concerns research problems in R N A structure, in the molecular recognition of structured R N A by diverse ligands, and in the mechanistic details of RNA's functional role following ligand binding. At the beginning of this new millennium, we celebrate the explosive development of exciting new tools and procedures whereby investigators explore R N A structure and function from the perspective of understanding RNA-ligand interactions. New insights into R N A processing are accompanied with improvements in older techniques as well as the dev
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