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JOURNAL OF CHROMATOGRAPHY LIBRARY - volume 45B
chromatography and modification of nucleosides part B: biological roles and function of modification
Chromatography and Modification of Nucleosides edited by C. W. Gehrke and K. C. T. Kuo Part A: Analytical Methods for Major and Modified Nucleosides HPLC, GC, MS, NMR, W a n d FT-IR Part 6: Biological Roles and Function of Modification Part C: Modified Nucleosides in Cancer and Normal Metabolism Methods and Applications Part D: Comprehensive Database for RNA and DNA Nucleosides Chemical, Biochemical, Physical, Spectral and Sequence
JOURNAL OF CHROMATOGRAPHY LIBRARY - volume 45B
chromatography and modification of nucleosides part B: biological roles and function of modification
edited by
Charles W. Gehrke and Kenneth C. T. Kuo Department of Biochemistry, University of Missouri-Columbia, and Cancer Research Center, P. 0. Box 1268, Columbia, MO 65205- 1268, U.S.A.
ELSEVIER Amsterdam
- Oxford - New York -Tokyo
1990
ELSEVIER SCIENCE PUBLISHERS B.V. Sara Burgerhartstraat25 P.O. Box 21 1, 1000 AE Amsterdam, The Netherlands Distributors for the United States and Canada: ELSEVIER SCIENCE PUBLISHING COMPANY INC. 655 Avenue of the Americas New York, NY 10010, U.S.A.
ISBN 0-444-88505-6
0 Elsevier Science Publishers B.V., 1990 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior written permission of the publisher, Elsevier Science Publishers B.V./ Physical Sciences & EngineeringDivision, P.O. Box 330, 1000 AH Amsterdam, The Netherlands. Special regulations for readers in the U.S.A. -This publication has been registered with the Copyright Clearance Center Inc. (CCC), Salem, Massachusetts. Information can be obtained from the CCC about conditions under which photocopies of parts of this publication may be made in the USA. All other copyright questions, including photocopying outside of the USA, should be referred to the publisher. No responsibility is assumed by the Publisher for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. Because of rapid advances in the medical sciences, the Publisher recommends that independent verification of diagnoses and drug dosages should be made. Although all advertising material is expected to conform to ethical (medical) standards, inclusion in this publication does not constitute a guarantee or endorsement of the quality or value of such product or of the claims made of it by its manufacturer. This book is printed on acid-free paper. Printed in The Netherlands
V
TABLE OF CONTENTS Preface
VII
A Dedication and Thanks
XI I
Special Acknowledgement to Dr. Robert W. Zumwalt -
XI I
Editors
-XI11
Contributors.
. XIX
Chromatography and Modification of Nucleosides: Parts A and C Introduction and Overview Chapter 1
Chapter 2
Chapter 3
Chapter 4
Chapter 5
Contents of
Dieter G. 5611.
-XLI I I B1
Synthesis and Function of Modified Nucleosides in tRNA Glenn R. Bj6rk and Jiirg Kohli
B13
Biosynthesis and Function of Queuine and Queuosine. Helga Kersten and Walter Kersten
669
Codon Usage and Q-Base Modification in Drosophila melanogaster. E. Kubli Solid Phase lmmunoassay for Determining the lnosine Content in Transfer RNA Edith F. Yamasaki, Altaf A. Wani and Ronald W. Trewyn Site Directed Replacement of Nucleotides in the Anticodon Loop of tRNA: Application