E-Book Overview
This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.
E-Book Content
Principles and Techniques of Biochemistry and Molecular Biology Seventh edition EDITED BY KEITH WILSON AND JOHN WALKER This new edition of the bestselling textbook integrates the theoretical principles and experimental techniques common to all undergraduate courses in the bio- and medical sciences. Three of the 16 chapters have new authors and have been totally rewritten. The others have been updated and extended to reflect developments in their field exemplified by a new section on stem cells. Two new chapters have been added. One on clinical biochemistry discusses the principles underlying the diagnosis and management of common biochemical disorders. The second one on drug discovery and development illustrates how the principles and techniques covered in the book are fundamental to the design and development of new drugs. In-text worked examples are again used to enhance student understanding of each topic and case studies are selectively used to illustrate important examples. Experimental design, quality assurance and the statistical analysis of quantitative data are emphasised throughout the book. • • • Motivates students by including cutting-edge topics and techniques, such as drug discovery, as well as the methods they will encounter in their own lab classes Promotes problem solving by setting students a challenge and then guiding them through the solution Integrates theory and practise to ensure students understand why and how each technique is used. K E I T H W I L S O N is Professor Emeritus of Pharmacological Biochemistry and former Head of the Department of Biosciences, Dean of the Faculty of Natural Sciences, and Director of Research at the University of Hertfordshire. J O H N W A L K E R is Professor Emeritus and former Head of the School of Life Sciences at the University of Hertfordshire. Cover illustration Main image Electrophoresis gel showing recombinant protein. Photographer: J.C. Revy. Courtesy of Science Photo Library. Top inset Transcription factor and DNA molecule. Courtesy of: Laguna Design/Science Photo Library. Second inset Microtubes, pipettor (pipette) tip & DNA sequence. Courtesy of Tek Image/Science Photo Library. Third inset Stem cell culture, light micrograph. Photographer: Philippe Plailly. Courtesy of Science Photo Library. Fourth inset Embryonic stem cells. Courtesy of Science Photo Library. Bottom inset Herceptin breast cancer drug, molecular model. Photographer: Tim Evans. Courtesy of Science Photo Library. Principles and Techniques of Biochemistry and Molecular Biology Seventh edition Edited by KEITH WILSON AND JOHN WALKER CAMBRIDGE UNIVERSITY PRESS Cambridge, New York, Melbourne, Madrid, Cape Town, Singapore, Sa˜o Paulo, Delhi, Dubai, Tokyo Cambridge