Gene Therapy Protocols METHODS IN MOLECULAR MEDICINE" J o h n M. Walker, SeRies EDzroR Gene Therapy Protocols, edited by Paul D. Robbins, 1997 Herpes Simplex Virus Protocols, edited by Moira S. Brown and Alasdair MacLean, 1997 Helicobacter Pylori Protocols, edited by Christopher L. Clayton and Harry T. Mobley, 1997 Lectin Methods and Protocols, edited by Jonathan M. Rhodes and Jeremy D. Milton, 1997 Human Cell Culture Protocols, edited by Gareth E. Jones, 1996 Antisense Therapeutics, edited by Sudhir Agrawal, 1996 Vaccine Protocols, edited by Andrew Robinson, Graham H. Farrar, and Christopher N. Wiblin, 1996 Prion Diseases, edited by Harry E Baker and Rosalind M. Ridley, 1996 Molecular Diagnosis of Cancer, edited by Finbarr Cotter, 1996 Molecular Diagnosis of Genetic Diseases, edited by Rob Elles, 1996 Gene Therapy Protocols Edited by Paul D. Robbins University of Pittsburgh, PA HumanaPress ~ Totowa, New Jersey © 1997 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular Medicine TM is a trademark of The Humana Press Inc. All authored papers, comments, opinions, conclusions, or recommendations are those of the author(s), and do not necessarily reflect the views of the publisher. This publication is printed on acid-free paper. ( ~ ANSI Z39.48-1984 (American Standards Institute) Permanence of Paper for Printed Library Materials. Cover illustration: Fig. 3 from Chapter 19, "Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy" by Sabine A. Eming and Jeffrey R. Morgan. For additional copies, pricing for bulk purchases, and/or information about other Humana titles, contact Humana at the above address or at any of the following numbers: Tel: 201-256-1699, Fax: 201-256-8341; E-mail:
[email protected] Photocopy Authorization Policy: Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Humana Press Inc., provided that the base fee of US $5.00 per copy, plus US $00.25 per page, is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923. For those organizations that have been granted a photocopy license from the CCC, a separate system of payment has been arranged and is acceptable to Humana Press Inc. The fee code for users of the Transactional Reporting Service is: [0-89603-307-4/97 $5.00 + $00.25]. Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1 Library of Congress Cataloging in Publication Data Main entry under title: Methods in molecular medicine TM. Gene therapy protocols/edited by Paul D. Robbins. p. cm.--(Methods in molecular medicine TM ) Includes index. ISBN 0-89603-307-4 (combbound) (alk. paper); ISBN 0-89603-484-4 (hardcover) (alk. paper) 1. Gene therapy--methodology. I. Robbins, Paul D. II. Series. [DNLM: 1. Gene therapy--methods. 2. Gene transfer. QZ 50 G3213 1997] RB 155.8.G465 1997 616'.042--dc20 DNLM/DLC for Library of Congress 96-42147 CIP Preface In the last few years, significant advances have been made in the area of gene therapy for both genetic and acquired diseases. Improvement in