E-Book Overview
Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In Steroid Receptor Methods: Protocols and Assays, a distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA-binding characteristics and hormone-binding assays and with the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness. Finally, a discussion by example presents the most current bioinformatics tools and analytical techniques. Timely and highly accessible, Steroid Receptor Methods: Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery.
E-Book Content
METHODS IN MOLECULAR BIOLOGY TM John M. Walker, Series Editor 188. Epithelial Cell Culture Protocols, edited by Clare Wise, 2002 187. PCR Mutation Detection Protocols, edited by Bimal D. M. Theophilus and Ralph Rapley, 2002 186. Oxidative Stress and Antioxidant Protocols, edited by Donald Armstrong, 2002 185. Embryonic Stem Cells: Methods and Protocols, edited by Kursad Turksen, 2002 18