Handbook Of Rna Biochemistry.

E-Book Overview

This all-in-one compendium of current laboratory techniques for the study of RNA brings together numerous novel methods that were hitherto only available through the primary literature. The editors - themselves experienced RNA researchers - have gathered expert authors from the US and Europe for the ultimate in everyday working knowledge. To this end, all the techniques covered are treated with the user in mind, including detailed practical advice and an invaluable "troubleshooting" section. The broad scope of the book allows the side-by-side treatment of specialized methods with basic biochemical techniques for the isolation, characterization and functional analysis of RNA. All pertinent laboratory methods are presented in detail, including RNA synthesis, modification and ligation Sequence and structure determination of RNA Isolation and purification of ribonucleoproteins RNA crystallization Single molecule studies on RNA Analysis of splice variants RNA imaging in vivo Modeling and prediction of RNA structure RNA microarrays Aptamer libraries and SELEX technology RNA interference In short, a guide to successful experimentation for molecular biologists, biochemists and biotechnologists and of interest to every researcher experimenting with RNA.

E-Book Content

Handbook of RNA Biochemistry Edited by R. K. Hartmann, A. Bindereif, A. Scho¨n, E. Westhof Handbook of RNA Biochemistry. Edited by R. K. Hartmann, A. Bindereif, A. Scho¨n, E. Westhof Copyright 8 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim ISBN: 3-527-30826-1 Further Titles of Interest U. Schepers RNA Interference in Practice 2005 ISBN 3-527-31020-7 G. Cesareni, M. Gimona, M. Sudol, M. Yaffe (Eds.) Modular Protein Domains 2005 ISBN 3-527-30813-X G. Kahl The Dictionary of Gene Technology, 3rd edition 2004 ISBN 3-527-30765-6 S. Brakmann, A. Schwienhorst (Eds.) Evolutionary Methods in Biotechnology 2004 ISBN 3-527-30799-0 S. Lorkowski, P. Cullen (Eds.) Analysing Gene Expression 2002 ISBN 3-527-30488-6 Handbook of RNA Biochemistry Edited by Roland K. Hartmann, Albrecht Bindereif, Astrid Scho¨n, Eric Westhof Editors Prof. Dr. Roland K. Hartmann Philipps-Universita¨t Marburg Institut fu¨r Pharmazeutische Chemie Marbacher Weg 6 35037 Marburg Germany [email protected]ff.uni-marburg.de Prof. Dr. Albrecht Bindereif Justus-Liebig-Universita¨t Giessen Institut fu¨r Biochemie Heinrich-Buff-Ring 58 35392 Giessen Germany [email protected] Dr. Astrid Scho¨n Universita¨t Leipzig Institut fu¨r Biochemie Bru¨derstr. 34 04103 Leipzig Germany [email protected] Prof. Dr. Eric Westhof CNRS – UPR 9002 Institut de Biologie Mole´culaire et Cellulaire 15 rue Rene´ Descartes 67084 Strasbourg France [email protected] 9 All books published by Wiley-VCH are carefully produced. Nevertheless, authors, editors, and publisher do not warrant the information contained in these books, including this book, to be free of errors. Readers are advised to keep in mind that statements, data, illustrations, procedural details or other items may inadvertently be inaccurate. Library of Congress Card No.: applied for A catalogue record for this book is available from the British Library. Bibl
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