E-Book Overview
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes* Extraction, subcellular fractionation, and solubilization procedures* Comprehensive purification techniques* Specialized purification procedures* Protein characterization* Immunological procedures* Computer analysis of protein structure
E-Book Content
Preface The explosion of work and interest in molecular biology in recent years has made protein purification something of a lost art, especially among younger biochemists and molecular biologists. At the same time, many of the more interesting biological problems have now reached a stage that requires work with purified proteins and enzymes. This has led to a situation in which many important studies stop at the demonstration of a physiological effect, and are not carried through to an understanding of the proteins responsible for the phenomenon. For these reasons a methods manual dealing with all aspects of protein purification should be a valuable addition to the Methods in Enzymology series and should be extremely useful to the scientific community. Although techniques for protein purification have been included in a few volumes in this series in the past, this Guide brings together in one source up-to-date procedures for purifying, characterizing, and working with proteins and enzymes. The volume begins with introductory chapters describing the