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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today - truly an essential publication for researchers in all fields of life sciences.Key Features* Promoter elements and RNA polymerase components* RNA polymerase and its subunits in prokaryotes* Biochemical assays of transcription initiation* RNA polymerase and associated factors from eukaryotes* Genetic analysis of transcription and its regulation
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METHODS IN ENZYMOLOGY EDITORS-IN-CHIEF John N. Abelson Melvin I. Simon DIVISION OF BIOLOGY CALIFORNIA INSTITUTE OF TECHNOLOGY PASADENA, CALIFORNIA FOUNDING EDITORS Sidney P. Colowick and Nathan O. Kaplan Contributors to Volume 371 Article numbers are in parentheses and following the names of contributors. Affiliations listed are current. Todd E. Adamson (19), Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 Yehudit Birger (39), Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4255 Michael Anikin (9), Morse Institute of Molecular Genetics, Department of Microbiology and Immunology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York 11203-2098 Sergei Borukov (16), Department of Microbiology and Immunology, SUNY Health Science Center at Brooklyn, 450 Clarkson Avenue Brooklyn, New York 11203-2098 Ekaterina Avetissova (13), Skirball Institute, New York University Medical Center,