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Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries.
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ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS JAACT'96 YOKOHAMA JAACT'96 ORGANIZING COMMITTEE Chairperson: KazuoNagai (Department of Bioengineering, Tokyo Institute of Technology) Secretary General: Masaaki Wachi (Department of Bioengineering, Tokyo Institute of Technology) Organizing Boards: Akio Ametani Shuichi Hashizume Kazuki Shin ohara (The University of Tokyo) (Morinaga Institute of Biological Science) (National Research Institute of Fisheries Science) (The University of Tokyo) (Kirin Brewery Co., Ltd.) Eiji Suzuki Takamoto Suzuki Program Committee Masuo Aizawa Toshihiro Akaike John Barford Shun'ichi Dosako Hiroyoshi Hoshi Wei-ShoulIn Koji Ikura David Barnes David Jayme Shuichi Kaminogawa Kin'ichi Kawamura Yasuo Kitagawa Yoji Mitsui Alan Moore Tadao Ohno Ralf Portner Ryuzo Sasaki Kazuo Shin Hajime Unno John Werenne Tokyo Institute of Technology Tokyo Institute of Technology University of Sydney Snow Brand Milk Products Co.,Ltd. Research Institute of Functional Peptides University of Minnesota Kyoto Institute of Technology Oregon State University Life Technologies Inc. The University of Tokyo Komatsugawa Chemical Engineering Co.,Ltd. Nagoya University Nat. Inst. Biosci. Human-Tech. Microbiological Associates Inc. Riken Gene Bank Technische Universitat Hamburg-Harburg Kyoto University Meiji Milk Products Co.,Ltd. Tokyo Institute of Technology Universite Libre de Bruxelles Animal Cell Technology: Basic & Applied Aspects Volume 9 Proceedings of the Ninth Annual Meeting of the Japanese Association for Animal Cell Technology, Yokohama, Japan, September 1-4, 1996 Edited by K. NAGAI Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan and M. WACHI Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan SPRINGER SCIENCE+BUSINESS MEDIA, B.V. A C.I.P. Catalogue record for this book is available from the Library of Congress. ISBN 978-94-010-6170-4 ISBN 978-94-011-5161-0 (eBook) DOl 10.1007/978-94-011-5161-0 Printed on acid-free paper All Rights Reserved © 1998 Springer Science+Business Media Dordrecht Originally published by Kluwer Academic Publishers in 1998 Softcover reprint of the hardcover lst edition 1998 No part of the material protected by this copyright notice may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording or by any information storage and retrieval system, without written permission frdm the copyright owner. Contents Preface xi I. Establishing new cell lines and characterizing molecular biological properties of cultured cells Stre