V O LU M E
F O U R T W E N T Y
METHODS
F I V E
IN
ENZYMOLOGY RNA Modification
METHODS IN ENZYMOLOGY Editors-in-Chief
JOHN N. ABELSON AND MELVIN I. SIMON Division of Biology California Institute of Technology Pasadena, California Founding Editors
SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
V O LU M E
F O U R T W E N T Y
METHODS
F I V E
IN
ENZYMOLOGY RNA Modification EDITED BY
JONATHA M. GOTT Center for RNA Molecular Biology Case Western Reserve University Cleveland, Ohio
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CONTENTS
Contributors Preface Volumes in Series
xi xv xvii
Section I. Modified Nucleotides
1
1. Identifying Modifications in RNA by MALD