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This latest volume of the biannual serial continues rapid, current coverage of all aspects of the molecular basis of human cancer, functions of oncogenes, and research strategies for cancer drug development and treatment. Topics reviewed in Volume 62 include chromosomes and cancer; pathways in Ras function; APC gene in human cancer; molecular cytogenetics of renal cell tumors; reverse transformation, genome exposure, and cancer; peptide-binding heat shock proteins in endoplasmic reticulum; new developments in the Epstein-Barr virus field; direct cellular communication and humoral immune response.

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ADVANCES IN CANCER RESEARCH VOLUME 62 This Page Intentionally Left Blank ADVANCES IN CANCERRESEARCH Edited by GEORGE F. VANDE WOUDE ABL-Basic Research Program NCI-Frederick Cancer Research and Development Center Frederick, Maryland GEORGE KLEIN Department of Tumor Biology Karolinska lnstitutet Stockholm, Sweden Volume 62 ACADEMIC PRESS, INC. A Division of liarcourt Brace 8 Company San Diego New York Boston London Sydney Tokyo Toronto This book is printed on acid-free paper. @ Copyright 0 1993 by ACADEMIC PRESS, INC. All Rights Reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Academic Press, Inc. 525 B Street, Suite 1900, San Diego, California 92101-4495 United Kingdom Edition published by Ac a de mic Press Limited 24-28 Oval Road, London NW1 7DX International Standard Serial Number: 0065-230X International Standard Book Number: 0- 12-006662-9 PRINTED IN THE UNITED STATES OF AMERICA 93 9 4 9 5 9 6 9 7 9 8 QW 9 8 1 6 5 4 3 2 1 CONTENTS CONTKIBUTOKS TO VOLUME 62 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Foundations in Cancer Research-Chromosomes The Evolution of an Idea PETER ix and Cancer: C. NOWELL I . Introduction . . . .. ............ ............... .SO) . . . . . . . . ............... 11. Boveri and Early (B 111. The Sternline Concept and Modern Cytogenetics IV. Early Findings in the Pre-banding Era (the 1960s) . . . . . . . . . . . . . . . . . . . V. Chromosome Banding and Clonal Evolution (the 1970s) . . . . . . . . . . . . . V I . Molecular Cytogenetics (the 1980s) .................... V l I . Conclusions . , . .. ................................. References ...................... .................... I 1 3 > * I 10 14 14 Pathways of Ras Function: Connections to the Actin Cytoskeleton GEORGE C. PRENDERCAST AND JACKSON B. GIBBS I. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11. Ras Guanine Nucleotide Exchange Factors . . . . . . . . . . . . . . . . . . . . . . . . . . 111. CAP and GAP-Associated Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IV. Signal Transduction Pathways Dttwnstream of Ras . . . . . . . . . . . . . . . . . . . V. Perspectives on Ras Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Note Added in P r o d . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v 19 20 29 35 415 .52 64 vi CONTENTS The Role of the Adenomatous Polyposis Coli (APC) Gene in Human Cancers YUSUKENAKAMURA I. Identification of the APC Gene . . . . . . , . , . , . . . . . . .