Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. <EM>Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, <EM>Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful <EM>Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, <EM>Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
METHODS
IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
METHODS
IN
MOLECULAR BIOLOGY
Bacteriophages Methods and Protocols, Volume 2 Molecular and Applied Aspects
Edited by
Martha R. J. Clokie University of Leicester, Leicester, UK
Andrew M. Kropinski Public Health Agency of Canada, Guelph, Ontario, Canada
TM
Editors Martha R. J. Clokie University of Leicester Leicester, UK
[email protected]
Andrew M. Kropinski Public Health Agency of Canada Guelph, Ontario, Canada Andrew
[email protected]
Series Editor John M. Walker University of Hertfordshire Hatfield, Herts UK
ISSN 1064-3745 ISBN 978-1-60327-564-4 DOI 10.1007/978-1-60327-565-1
e-ISSN 1940-6029 e-ISBN 978-1-60327-565-1
Library of Congress Control Number: 2008939449 c 2009 Humana Press, a part of Springer Science+Business Media, LLC All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper springer.com
Preface We are increasingly aware of the many and varied roles that bacteriophages play in microbial ecology and evolution. The implications of bacteriophage–bacteria interactions range from the evolution of pathogenicity to oceanic carbon cycling. However, working with bacteriophages can be difficult due to their small size and specific bacterial host requirements. Written by top international bacteriophage researchers, these volumes pull together a vast body of know