The Zebrafish: Cellular And Developmental Biology, Part A (methods In Cell Biology, Volume 100)

Series Editors Leslie Wilson Department of Molecular, Cellular and Developmental Biology University of California Santa Barbara, California Paul Matsudaira Department of Biological Sciences National University of Singapore Singapore Methods in Cell Biology VOLUME 100 The Zebrafish: Cellular and Developmental Biology, Part A Third Edition Edited by H. William Detrich III Department of Biology, Northeastern University, Boston, MA, USA Monte Westerfield Institute of Neuroscience, University of Oregon, Eugene, OR, USA Leonard I. Zon Division of Hematology/Oncology, Children's Hospital of Boson, Department of Pediatrics and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA AMSTERDAM • BOSTON • HEIDELBERG • LONDON NEW YORK • OXFORD • PARIS • SAN DIEGO SAN FRANCISCO • SINGAPORE • SYDNEY • TOKYO Academic Press is an imprint of Elsevier Academic Press is an imprint of Elsevier 30 Corporate Drive, Suite 400, Burlington, MA 01803, USA 525 B Street, Suite 1900, San Diego, CA 92101-4495, USA 32, Jamestown Road, London NW1 7BY, UK Linacre House, Jordan Hill, Oxford OX2 8DP, UK Third edition 2010 Copyright © 2010 Elsevier Inc. All rights reserved No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, mechanical, photocopying, recording or otherwise without the prior written permission of the publisher Permissions may be sought directly from Elsevier’s Science & Technology Rights Department in Oxford, UK: phone (+44) (0) 1865 843830; fax (+44) (0) 1865 853333; email: [email protected] Alternatively you can submit your request online by visiting the Elsevier web site at http://elsevier.com/locate/permissions, and selecting Obtaining permission to use Elsevier material Notice No responsibility is assumed by the publisher for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. Because of rapid advances in the medical sciences, in particular, independent verification of diagnoses and drug dosages should be made ISBN–13: 978-0-12-384892-5 ISSN: 0091-679X For information on all Academic Press publications visit our website at elsevierdirect.com Printed and bound in USA 10 11 12 13 10 9 8 7 6 5 4 3 2 1 Working together to grow libraries in developing countries www.elsevier.com | www.bookaid.org | www.sabre.org DEDICATION We dedicate the Third Edition of Methods in Cell Biology: The Zebrafish to Wolfgang Driever, Mark C. Fishman, Charles Kimmel, and Christiane Nüsslein-Volhard. Through their foresighted embrace of the zebrafish as a model vertebrate and their pursuit of genetic screens to illuminate vertebrate development, they fostered the emergence of the v