Current Topics In Membranes & Transport Vol. 23: Genes & Proteins: Transport Proteins & Receptors

C u rrent Topics in Membranes and Transport VOLUME 23 Genes and Membranes: Transport Proteins and Receptors Advisory Board M . P. Blaustein G. Blobel J. S. Cook P . A . Knauf Sir H. L. Kornberg C. A. Pasternak W . D. Stein W . Stoeckenius K. 3. Ullrich Contributors Giovanna Ferro-Luzzi Ames Sandra Barbel Sylvie Bedu Alan Boyd William A . Catterall Maria Costa Carolyn Doyle Wolfgang Epstein Mary-Jane Gething Tohru Gonoi Lily Yeh Jan Yuh Nung Jan Alexandra Krikos Ching Kung Cheryl Laffer Robert Levenson Benno Muller-Hill Norihiro Mutoh Patrick O’Farrell Resha M . Putzrath Michael Roth Yoshiro Saimi Lawrence Salkoff Joe Sambrook A . E . Senior Donna Seto-Young Melvin I . Simon Leslie Timpe T . Hastings Wilson C u rrent Topics in Membranes and Transport Edited by Felix Bronner Arnost Kleinzeller Department of Oral Biology Universitj of Connecticut Health Center Farmington. Connecticut Department of Physiology University o j Pennsylvania School Philadelphia, Pennsylvania of Medicine VOLUME 23 Genes and Membranes: Transport Proteins and Receptors Guest Editors Edward A. Adelberg Carolyn W. Slayman Department of Human Genetics Yale Universini School of Medicine New Haven. Connecticut Departments of Human Genetics and Physlolog?; Yale University School of Medicine New Haven. Connecticut Volume 23 is part of the series (p. xiii) from the Yale Department of Physiology under the editorial supervision of: Joseph F. Hoffman Gerhard Giebisch Department of Physiology Yale University School of Medicine New Haven, Connecticul Deparfmenf qf Physiology Yale Universip School of Medicine New Haven, Connecricur 1985 m ACADEMIC PRESS, INC. (Harcoun Brace Jovanovich, Publishers) Orlando San Diego New York London Toronto Montreal Sydney Tokyo COPYRIGHT @ 1985, BY ACADEMIC PRESS, INC. ALL RIGHTS RESERVED. NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM OR BY ANY MEANS, ELECTRONIC OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT PERMISSION IN WRITING FROM THE PUBLISHER. ACADEMIC PRESS, INC. Orlando. Florida 32887 United Kingdom Edition published by ACADEMIC PRESS INC. (LONDON) LTD. 24-28 Oval Road, London NWI 7DX LIBRARY OF CONGRESS CATALOG CARD 1 SBN 0-12- 153323-9 PRINTED IN THE UNITED STATES OF AMERICA 85 86 81 88 9 8 7 65 4 3 2 I NUMBER:7 0- 1 1 7091 Contents Contributors, ix Preface, xi Yale Membrane Transport Processes Volumes, xiii RECEPTORS AND RECOGNITION PROTEINS PART I . CHAITER 1. Sensory Transduction in Bacteria MELVIN I. SIMON, ALEXANDRA KRIKOS, NORIHIRO MUTOH. AND ALAN BOYD I. 11. 111. IV. Introduction, 3 Mapping the Transducer Genes, 7 The Amino Acid Sequences of Three Transducers, 8 A Model of the Structure of the Sensory Transducers, 11 References. 15 CHAPTER 2. Mutational Analysis of the Structure and Function of the Influenza Virus Hemagglutinin MARY-JANE GETHING, CAROLYN DOYLE, MICHAEL ROTH, AND JOE SAMBROOK I. Introduction, 17 11. Expression of Wild-Type HA in Simian Cells Using a Recombinant SV40 Viral Vector, 19 111. Analysis of the Expression of Mutant HA Proteins, 22 References. 38 V CONTENTS vi CHANNELS PART 11. CHAPTER 3. Ca*+ Channels of Paramecium: A Multidisciplinary Study CHING KUNG AND YOSHIRO SAIMI I . A Swimming Neuron, 46 11. Ion Channels, 47 111. Mutants, 50 IV. Pawns and CNRs, 51 V. Curing Factors, 53 VI. Dancer Mutants, Possi