E-Book Overview
Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.
E-Book Content
Protein Expression
The Practical Approach Series SERIES EDITOR B. D. HAMES School of Biochemistry and Molecular Biology University of Leeds, Leeds LS2 9JT, UK
See also the Practical Approach web site at http://www.oup.co.uk/PAS * indicates new and forthcoming titles Affinity Chromatography Affinity Separations Anaerobic Microbiology Animal Cell Culture (2nd edition) Animal Virus Pathogenesis Antibodies I Antibodies II Antibody Engineering * Antisense Technology Applied Microbial Physiology Basic Cell Culture Behavioural Neuroscience Bioenergetics Biological Data Analysis Biomechanics—Materials Biomechanics—Structures and Systems Biosensors Carbohydrate Analysis (2nd edition) Cell-Cell Interactions The Cell Cycle Cell Growth and Apoptosis
if Cell Separation Cellular Calcium Cellular Interactions in Development Cellular Neurobiology * Chromatin if Chromosome Structural Analysis Clinical Immunology Complement if Crystallization of Nucleic Acids and Proteins (2nd edition) Cytokines (2nd edition) The Cytoskeleton Diagnostic Molecular Pathology I Diagnostic Molecular Pathology II DNA and Protein Sequence Analysis DNA Cloning 1: Core Techniques (2nd edition) DNA Cloning 2: Expression Systems (2nd edition) DNA Cloning 3: Complex Genomes (2nd edition)
DNA Cloning 4: Mammalian Systems (2nd edition) * Drosophila (2nd edition) Electron Microscopy in Biology Electron Microscopy in Molecular Biology Electrophysiology Enzyme Assays Epithelial Cell Culture Essential Developmental Biology Essential Molecular Biology I Essential Molecular Biology II * Eukaryotic DNA Replication Experimental Neuroanatomy Extracellular Matrix Flow Cytometry (2nd edition) Free Radicals Gas Chromatography Gel Electrophoresis of Nucleic Acids (2nd edition) * Gel Electrophoresis of Proteins (3rd edition) Gene Probes 1 Gene Probes 2 Gene Targeting Gene Transcription * Genome Mapping Glycobiology if Growth Factors and Receptors Haemopoiesis if High Resolution Chromotography Histocompatibility Testing HIV Volume 1 HIV Volume 2
* HPLC of Macromolecules (2nd edition) Human Cytogenetics I (2nd edition) Human Cytogenetics II (2nd edition) Human Genetic Disease Analysis if Immobilized Biomolecules in Analysis Immunochemistry 1 Immunochemistry 2 Immunocytochemistry if In Situ Hybridization (2nd edition) lodinated Density Gradient Media Ion Channels if Light Microscopy (2nd edition) Lipid Modification of Proteins Lipoprotein Analysis Liposomes Mammalian Cell Biotechnology Medical Parasitology Medical Virology MHC Volume 1 MHC Volume 2 * Molecular Genetic Analysis of Populations (2nd edition) Molecular Genetics of Yeast Molecular Imaging in Neuroscience Molecular Neurobiology Molecular Plant Pathology I Molecular Plant Pathology II Molecular Virology Monitoring Neuronal Activity
Mutagenicity Testing * Mutation Detection Neural Cell Culture Neural Transplantation Neurochemistry (2nd edition) Neuronal Cell Lines NMR of Biological Macromolecules Non-isotopic Methods in Molecular Biology Nucleic Acid Hybridisation Oligonucleotides and Analogues Oligonucleotide Synthesis PCR 1 PCR 2 * PCR 3:PCR In Situ Hybridization Peptide Antigens Photosynthesis: Energy Transduction Plant Cell Biology Plant Cell Culture (2nd edition) Plant Molecular Biology Plasmids (2nd editio