E-Book Overview
A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.
E-Book Content
M E T H O D S I N M O L E C U L A R M E D I C I N E TM Myeloid Leukemia Methods and Protocols Edited by Harry Iland Mark Hertzberg Paula Marlton Myeloid Leukemia M E T H O D S I N M O L E C U L A R M E D I C I N E™ John M. Walker, SERIES EDITOR 125 Myeloid Leukemia: Methods and Protocols, 125. edited by Harry Iland, Mark Hertzberg, and Paula Marlton, 2006 124 Magnetic Resonance Imaging: Methods and 124. Biological Applications, edited by Pottumarthi V. Prasadi, 2006 123. 123 Marijuana and Cannabinoid Research: Methods and Protocols, edited by Emmanuel S. Onaivi, 2006 122. 122 Placenta Research Methods and Protocols: Volume 2, edited by Michael J. Soares and Joan S. Hunt, 2006 121. 121 Placenta Research Methods and Protocols: Volume 1, edited by Michael J. Soares and Joan S. Hunt, 2006 120. 120 Breast Cancer Research Protocols, edited by Susan A. Brooks and Adrian Harris, 2005 119. Human Papillomaviruses: Methods and 119 Protocols, edited by Clare Davy and John Doorbar, 2005 118 118. Antifungal Agents: Methods and Protocols, edited by Erika