M E T H O D S I N M O L E C U L A R B I O L O G Y™ DNA Viruses Methods and Protocols Edited by Paul M. Lieberman The Wistar Institute, Philadelphia, PA © 2005 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512 www.humanapress.com All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular BiologyTM is a trademark of The Humana Press Inc. All papers, comments, opinions, conclusions, or recommendations are those of the author(s), and do not necessarily reflect the views of the publisher. This publication is printed on acid-free paper. ∞ ANSI Z39.48-1984 (American Standards Institute) Permanence of Paper for Printed Library Materials. Cover design by Patricia F. Cleary Cover illustrations: Icosahedral orientation convention and masking of the cryo-EM map (foreground; Fig. 3, Chap. 8; see full caption and discussion on p. 115). Pseudovirion infection assay with HPV33 (background; Fig. 4, Chap. 14; see full caption on p. 208 and discussion on pp. 205–206). For additional copies, pricing for bulk purchases, and/or information about other Humana titles, contact Humana at the above address or at any of the following numbers: Tel.: 973-256-1699; Fax: 973-256-8341; E-mail:
[email protected]; or visit our Website: www.humanapress.com Photocopy Authorization Policy: Authorization to photocopy items for internal or personal use, or the internal or personal use of specific clients, is granted by Humana Press Inc., provided that the base fee of US $25.00 per copy is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923. For those organizations that have been granted a photocopy license from the CCC, a separate system of payment has been arranged and is acceptable to Humana Press Inc. The fee code for users of the Transactional Reporting Service is: [1-58829-353-X/05 $25.00 ]. Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1 eISBN 1-59259-848-X ISSN 1064-3745 Library of Congress Cataloging-in-Publication Data DNA viruses : methods and protocols / edited by Paul M. Lieberman. p. ; cm. -- (Methods in molecular biology ; 292) Includes bibliographical references and index. ISBN 1-58829-353-X (hardcover : alk. paper) 1. DNA viruses--Laboratory manuals. [DNLM: 1. DNA Viruses. 2. Genetic Techniques. 3. Virology--methods. QW165 D6288 2005] I. Lieberman, Paul M. II. Series: Methods in molecular biology (Clifton, N.J.) ; v. 292. QR394.5.D634 2005 579.2'4--dc22 2004012488 Preface The application of modern methods in molecular biology and biotechnology to the study of human, animal, and plant viruses continues to revitalize the age-old discipline of virology. Modern virology remains at the vanguard of contemporary biomedical research largely owing to the impact of viruses in human disease and pathogenesis, but also because of the utility of v