E-Book Overview
In the decade since the publication of the bestselling first edition, Polymerase Chain Reaction (PCR) technology has been at the forefront of dramatic developments in biochemistry. Many exciting advances have been made in this specialized field of DNA analysis and a large number of commercial kits and additives are now available. There has never been a better time for a comprehensive resource covering the current range of PCR strategies and uses.PCR Technology: Current Innovations, Second Edition is a fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. It provides theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. The book begins with discussions of the preparation of PCR experiments, followed by examples of analytical PCR divided into qualitative and quantitative applications. The final section explores preparative methods addressing DNA generation for further analysis and in vitro evolution.Featuring detailed presentations of PCR protocols, this volume contains critical information for practitioners in a wide variety of fields, including forensics, molecular biology research, clinical DNA diagnostics, botany, and paleontology. It provides the essential tools for developing innovative approaches in this leading technology of the genomics revolution.
E-Book Content
S E C O N D E D I T I O N PCR TECHNOLOGY Current Innovations 1184_C00.fm Page ii Wednesday, October 15, 2003 1:05 PM S E C O N D E D I T I O N PCR TECHNOLOGY Current Innovations EDITED BY Thomas Weissensteiner, Hugh G. Griffin, and Annette Griffin CRC PR E S S Boca Raton London New York Washington, D.C. 1184_C00.fm Page iv Wednesday, October 15, 2003 1:05 PM Library of Congress Cataloging-in-Publication Data PCR technology : current innovations / edited by Thomas Weissensteiner, Hugh G. GrifÞn, and Annette GrifÞn.—2nd. ed. p. cm. Includes bibliographical references and index. ISBN 0-8493-1184-5 1. Polymerase chain reaction—Methodology. I. Weissensteiner, Thomas. II. GrifÞn, Hugh G. III. GrifÞn, Annette M. QP606.D46P362 2003 572.8¢636—dc22 2003058465 This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use. Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, microÞlming, and recording, or by any information storage or retrieval system, without prior permission in writing from the publisher. All rights reserved. Authorization to photocopy items for internal or personal use, or the personal or internal use of speciÞc clients, may be granted by CRC Press LLC, provided that $1.50 per page photocopied is paid directly to Copyright clearance Center, 222 Rosewood Drive, Danvers, MA 01923 USA. The fee code for users of the Transactional Reporting Service is ISBN 0-8493-1184-5/04/$0.00+$1.50. The fee is subject to change without notice. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. The consent of CRC Press LLC does not extend to copying for general distribution, for promotion, for creating new works, or for resale. SpeciÞc permission must be obtained in writing from CRC Press LLC for such copying. Direct all inquiries to CRC Press LLC, 2000 N.W. Corporate Blvd., Boca