E-Book Overview
BD Biosciences is pleased to introduce the new Techniques for Immune Function Analysis, Application Handbook 1st Edition. This handbook grew out of the original Cytokine/ Chemokine Application Manual that was first published in 1997.
E-Book Content
Techniques for Immune Function Analysis Application Handbook 1st Edition BD Biosciences For additional information please access the Immune Function Homepage at www.bdbiosciences.com/immune_function For Research Use Only. Not for use in diagnostic or therapeutic procedures. Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Becton Dickinson and Company is strictly prohibited. All applications are either tested in-house or reported in the literature. See Technical Data Sheets for details. BD, BD Logo and all other trademarks are the property of Becton, Dickinson and Company. ©2003 BD Table of Contents Preface ....................................................4 Chapter 1: Immunofluorescent Staining of Cell Surface Molecules for Flow Cytometric Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Chapter 2: BD™ Cytometric Bead Array (CBA) Multiplexing Assays . . . . . . 35 Chapter 3: BD™ DimerX MHC:Ig Proteins for the Analysis of Antigen-specific T Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Chapter 4: Immunofluorescent Staining of Intracellular Molecules for Flow Cytometric Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Chapter 5: BD FastImmune™ Cytokine Flow Cytometry . . . . . . . . . . . . . . . . 85 Chapter 6: BD™ ELISPOT Assays for Cells That Secrete Biological Response Modifiers . . . . . . . . . . . . . . . . .