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Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual.
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Methods in Molecular Biology TM TM VOLUME 117 Electron Microscopy Methods and Protocols Edited by M. A. Nasser Hajibagheri HUMANA PRESS Preparation and Staining of Sections 1 1 General Preparation of Material and Staining of Sections Heather A. Davies 1. Introduction This chapter is aimed at those who have not previously done any processing for electron microscopy (EM). It deals with basic preparation of many different types of mammalian material for ultrastructural examination; for processing of plant material (see Hall and Hawes, ref. 1). The material to be processed may be cell suspensions, particulates, monolayer cultures, or tissue derived from organs. The former three must initially be processed differently from the latter. For EM, the ultrastru