E-Book Overview
Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Patch-Clamp Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.
E-Book Content
Methods in Molecular Biology 1183 Marzia Martina Stefano Taverna Editors Patch-Clamp Methods and Protocols Second Edition METHODS IN M O L E C U L A R B I O LO G Y Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Patch-Clamp Methods and Protocols Second Edition Edited by Marzia Martina Department of Translational Bioscience, National Research Council of Canada, Ottawa, ON, Canada Stefano Taverna Department of Neuroscience & Brain Technologies, Italian Institute of Technology, Genoa, Italy Editors Marzia Martina Department of Translational Bioscience National Research Council of Canada Ottawa, ON, Canada Stefano Taverna Department of Neuroscience & Brain Technologies Italian Institute of Technology Genoa, Italy ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-1095-3 ISBN 978-1-4939-1096-0 (eBook) DOI 10.1007/978-1-4939-1096-0 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014942964 © Springer Science+Business Media New York 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. P