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In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In <EM>Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful <EM>Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and groundbreaking, <EM>Micro and Nano Technologies in Bioanalysis: <EM>Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.
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METHODS IN MOLECULAR BIOLOGY™ Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For other titles published in this series, go to www.springer.com/series/7651 METHODS IN MOLECULAR BIOLOGY™ Micro and Nano Technologies in Bioanalysis Methods and Protocols Edited by James Weifu Lee and Robert S. Foote Oak Ridge National Laboratory, Oak Ridge, TN, USA Editors James Weifu Lee Oak Ridge National Laboratory Oak Ridge, TN, USA Robert S. Foote Oak Ridge National Laboratory Oak Ridge, TN, USA ISSN: 1064-3745 e-ISSN: 1940-6029 ISBN: 978-1-934115-40-4 e-ISBN: 978-1-59745-483-4 DOI: 10.1007/978-1-59745-483-4 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009929345 © Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is for-bidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with re-spect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Preface This book provides current information on the development of microfluidics, nanotechnologies, and physical science techniques for the separation, detection, manipulation, and analysis of biomolecules, and should be useful to a wide audience, including molecular and cell biologists, biochemists, microbiolog