E-Book Overview
It is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research. Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease.
E-Book Content
Stress Response METHODS IN MOLECULAR BIOLOGY" John M. Walker, SERIESEDITOR 99. Stress Response: Mefhods ond Profocols, edited by Stephen M. Kepe, 2000 98. Forensic DNA ProfilingProtocols, edited by forrick J. Lincoln and Jomes M. Thomson, 1998 97. Molecular Embryology: Mefhods and Prorocols. edited by f o u l T. Shorpe and lvor Mason, 1999 96. Adhesion Protein Protocols, edited by Elisobeffo Dejono ond Mottico Coroda, 1999 95. DNA Topoisomerases Protocols: 11. Enzymolo~~ ond Drugs. edited by M a y A n n Bjornsfi and Neil Osherofi 2000 94. DNA Topoisomerases Protocols: 1. DNA Topology ond Engvnes. edited by Mow-Ann Bjornsfiond Neil Osheroff, 1999 93. Protein PhosphataseProtocols,editedbyJohn K Ludlow, 1998 92. PCR i n Bioanalysis, edited by Stephen J. Melfzer, 1998 91. Flow Cytometry Protocols. edited by Mork J. Joroszeski, Richard Heller. and Richord Gilbert. 1998 90. DrueDNA interaction Protocols, edited by Keith R. Fox. 1998 89. Retinoid Protocols, edited by Christopher Redfern. 1998 88. Protein TargetingProtocols, edited by RogerA. Clegg, 1998 87. Combinatorial Peptide Library Protocols, edited by Shmuel Cobi/!v, 1998 86. RNA Isolation and Characterization Protocols, edited by Rolph Ropley and Dovid L. Monning, 1998 85. Differential Display Methods and Protocols, edited by feng Liongand Arfhur B. fardee. 1997 84. Transmembrane Signaling Protocols, editedby 0 0 1 0Bor-Sogi, 1998 83. Receptor Signal Transduction Protocols, edited by R. A. John cllolliss. 1997 82.Arabidopsis Protocols, edited by Josi M Morfinez-Zopoter ond Julio Salinos. 1998 81. Plant VirologyProtocols: From Virus Isolationto Transgenic Resisfonce, edited by C o w D. Fosfer and Sol!v Toylor, 1998 80.lmmunochemicalProtocols(2nd. ed.), editedby John Pound, 1998 79. Polyamine Protocols, edited by Dovid M.L. Morgon. 1998 78. Antibacterial Peptlde Protocols, edited by William M. Shofr, 1997 77. Protein Synthesis: Mefhods ond Profocols. edited by Robin Marfin. 1998 76. Glycoanalysis Protocols (2nd. ed.), edited by Elizobefh F. Hounsell, 1998 75. Basic Cell Culture Protocols(2nd. ed.), edited by Jefrey W. 74. 73. 72. 71. 70. 69. Pollard ond John M. Walker, l997 Ribozyme Protocols, edited by Philip C. Turner, 1997 Neuropeptide Protocols, editedby G. Brent /wine ond Corvell H. Williams. 1997 Neurotransmitter Methods, edited by Richord C. Royne. 1997 PRINS and In Sifu PCR Protocois, edited by John R. Cosden. 1996 Sequence Data Analysis Guidebook, edited by Simon R. Swindell. 1997 cDNALibraryProtocols,editedby Ion