Advances in MICROBIAL PHYSIOLOGY This Page Intentionally Left Blank Advances in MICROBIAL PHYSIOLOGY edited by A. H. ROSE J. GARETH MORRIS School of Biological Sciences Bath University England Department of Botany and Microbiology University College of Wales Aberystwyth, Wales D. W. TEMPEST Laboratorium voor Microbiologie Universiteit van Amsterdam The Netherlands Volume 24 1983 ACADEMIC PRESS, INC. (Harcourt Brace lovanovich, Publishers) London Orlando San Diego New York Toronto Montreal Sydney Tokyo ACADEMIC PRESS INC. (LONDON) LTD. 24/28 Oval Road London NW 1 7DX United States Edition published by ACADEMIC PRESS, INC. Orlando, Florida 32887 Copyright 0 1983 by ACADEMIC PRESS INC. (LONDON) LTD. AN Rights Reserved No part of this book may be reproduced in any form by photostat, microfilm, or any other means, without the written permission from the publishers ISBN &12-027724-7 ISSSN 0065 291 1 PRINTED I N T H E UNITED STATES OFAMERICA 85 86 87 88 9 8 7 6 5 4 3 2 Contributors J. P. van DIJKEN Laboratory of Microbiology, Delft University of Technology, P 0 Box 5 , Delft, The Netherlands A. A. GUFFANTI Mount Sinai School of Medicine of the City University of New York, New York 10029, USA W . HARDER Department of Microbiology, University of Groningen, Broerstraat 5 , Groningen, The Netherlands A. L. KOCH Department of Biology, Indiana University, Bloomington, Indiana 47405, USA T . A. KRULWICH Mount Sinai School of Medicine of the City University of New York, New York 10029, USA I. S. KULAEV Institute of Biochemistry and Physiology of Micro-organisms, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR V. M . VAGABOV Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR M . VEENHUIS Laboratory of Electron Microscopy, University of Groningen, Broerstraat 5, Groningen, The Netherlands J. G. ZEIKUS Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA This Page Intentionally Left Blank Contents The Significance of Peroxisomes in One-Carbon Compounds in Yeasts J . P. VAN DIJKEN and W . HARDER I . Introduction . . . . . . . I1. Role of peroxisomes in methanol metabolism the Metabolism of by M. VEENHUIS. . . . . . . . . . . . . . . . . . . . . . . A . Dissimilation of methanol . . . B. Assimilation of methanol . . . . C . Structure and function of peroxisomes . . . . . D . The molecular substructure of crystalline peroxisomes . . I11. Role of peroxisomes in methylated amine metabolism . . . . A . Methylated amines as a nitrogen source . . . . . . B. Peroxisomes and the metabolism of methylated amines . . . C . Peroxisomes involved in the concurrent metabolism of Carbon- and Nitrogen-sources . . . . . . . . . . IV . Biogenesis of peroxisomes . . . . . . . . . A . Regulation of the synthesis of peroxisomal enzymes. . . . B. Development of peroxisomes during vegetative growth . . . C . Development and function of peroxisomes during spore formation D . Assemblage of peroxisomes . . . . . . . . V. Inactivation of peroxisomal enzymes and degradation of peroxisomes . A . Regulation of enzyme activity by inactivation . . . . . B. Modification inactivation of peroxisomal enzymes . . . . C . Degradative inactivation of peroxisomal enzymes . . . . D . Subcellular events in peroxisomal degradation . . . . . VI . Concluding remarks . . . . . . . . . . VII . Acknowledgement . . . . . . . . . . References . . . . . . . . . . . . 2 5 6 13 16 24 30 30 31 37 40 41 47 53 55 58 58 60 64 69 76 76 76 Polyphosphate Metabolism in Micro-Organisms by IGOR S. KULAEV and VLADlM