Cellular Mechanisms Of Renal Tubular Ion Transport

Current Topics in Membranes and Transport Volume 13 Cellular Mechanisms of Renal Tubular Ion Transport Advisory Board I . S. Edelman Alvin Essig Franklin M . Harold James D. Jamieson Anthony Martonosi Shmuel Razin Martin Rodbell Aser Rothstein Stanley G. Schultz Contributors Rolf Kinne Peter S. Aronson E. Kinne-Saffran M . Barac Kunihiko Kotera Richard Bauer Takahiro Kubota Franz Beck Simon A. Lewis Reinier Beeuwkes III Bruce Biagi G. Malnic Jean- Ph ilippe Bonjour June Mason Walter F. Boron Yutaka Matsumura Emile L . Boulpaep John W . Mills F. Morel S. S. Campiglia Heini Murer D. ChabardPs Mortimer M. Civan Margarita PPrez-Gonzalez V . L . Costa Silva de la Manna Fulgencio Proverbio Joel DeLong M . de Mello Aires Luis Reuss Donald R. DiBona Roger Rick Adolf Dorge Clara V . Riddle Thomas P. Dousa Paula Rogenes Douglas C. Eaton Christiane Roloff Stephen A . Ernst Seymour Rosen Carla Evers Henry Sackin Arthur L. Finn Bertram Sacktor Herbert Fleisch Kenneth R . Spring Mamoru Fujimoto Reinhard Stoll Gerhard Giebisch R . C. Thomas Ernest0 Gonzalez Klaus Thurau M . Imbert-Teboul James B. Wade Karl J . Karnaky, Jr. Dan J . Welling Michael Kashgarian Larry W . Welling Raja N . Khuri Guillermo Whittembury Genjiro Kimu ra Nancy K . Wills Thomas Zeuthen Current Topics in Membranes and Transport Edited by Felix Bronner Arnost Kieinzeiler Department of Oral Biology University of Connecticut Health Center Farmington, Connecticut Department of Physiology University of Pennsylvania School of Medicine Philadelphia, Pennsylvania VOLUME 13 Cellular Mechanisms of Renal Tubular Ion Transport Guest Editor Emile L. Boulpaep Department of Physiology Yale University School of Medicine N e w Haven. Connecticut Volume 13 is part of the series (p. xxi) from the Yale Department of Physiology under the editorial supervision of: Joseph F. Hoffman Gerhard Giebirch Department of Physiology Yale University School of Medicine N e w Haven. Connecticut Department of Physiology Yale University School of Medicine N e w Haven, Connecticut 1980 New York London Toronto Sydney San Francisco COPYRIGHT @ 1980, BY ACADEMIC PRESS, INC. ALL RIGHTS RESERVED. NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM OR BY ANY MEANS, ELECTRONIC OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT PERMISSION IN WRITING FROM THE PUBLISHER. ACADEMIC PRESS, INC. 1 1 1 Fifth Avenue, New York, New York 10003 United Kingdom Edition published by ACADEMIC PRESS, INC. ( L O N D O N ) LTD. 24/28 Oval Road, London NW1 LIBRARY OF 7DX CONGRESS CATALOG CARD NUMBER: 70-1 17091 ISBN 0- 12- 1533 13-1 PRINTED IN THE UNITED STATES OF AMERICA 80 81 82 83 9 8 7 6 5 4 3 2 1 Contents List of Contributors, xi11 Foreword, xvll Preface, xlx Yale Membrane Transport Processes Volumes, xxl Contents of Previous Volumes, xxlii ION ACTIVITY AND ELEMENTAL COMPOSITION OF INTRAEPITHELIAL COMPARTMENTS PART I. CHAPTER I. intracelluiar pH Regulation WALTER F. BORON I. Measurement and Normal Values of pH,, 4 11. Factors Influencing pHi, 6 111. Cellular Regulation of PHI, 10 IV. Summary, 20 References, 20 CHAPTER 2. Reversal of the pH,-Regulating System in a Snali Neuron R. C. THOMAS I. 11. 111. IV. Introduction, 23 Methods, 24 Results, 25 Discussion, 28 References, 28 CHAPTER 3. How to Make and Use Double-Barreled Ion-Selective Mlcroeiectrodes THOMAS ZEUTHEN 1. 11. 111. IV. V. Introduction, 31 Construction of Electrodes, 33 Selection of Results, 37 The Effects of Leaks, 38