E-Book Overview
Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the RNA and protein levels-and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.
E-Book Content
Methods in Molecular Biology
TM
VOLUME 129
Integrin Protocols Edited by
Anthony Howlett
HUMANA PRESS
Phage Libraries of Integrin-Binding Peptides
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1 Integrin-Binding Peptides Derived from Phage Display Libraries Erkki Koivunen, Bradley H. Restel, Daniel Rajotte, Johanna Lahdenranta, Martin Hagedorn, Wadih Arap, and Renata Pasqualini 1. Introduction 1.1. Use of Phage Display Libraries in Integrin Research Integrins are a family of cell-surface receptors that recognize extracellular matrix proteins and mediate cell adhesion. Certain integrins bind to proteins containing the sequence Arg-Gly-Asp (RGD; ref. 1). Such RGD-binding integrins include _5`1, _v`3, _v`5, and _IIb`3 (2,3). Peptide libraries displayed on filamentous phage have provided a straightforward method to characte