E-Book Overview
This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
E-Book Content
Preface This Methods in Enzymology volume deals with the rapidly evolving topic of confocal microscopy. The OVID database (including MEDLINE, Current Contents, and other sources) lists 76 references to confocal microscopy for the five-year period 1985-1989. In contrast, for the four-year period 1995-1998, nearly 3600 references are listed. This volume documents many diverse uses for confocal microscopy in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in the sources from which they were taken. The techniques are described in a context that allows comparisons to other related methodologies. The authors were encouraged to do this in the belief that such comparisons are valuable to readers who must adapt extant procedures to new systems. Also, so far as possible, methodologies are presented in a manner that stresses their general applicability and potential limitations. Although for various reaso