E-Book Overview
This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.
E-Book Content
Methods in Molecular Biology 1250
Mathieu Vinken Vera Rogiers Editors
Protocols in In Vitro Hepatocyte Research
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Protocols in In Vitro Hepatocyte Research Edited by
Mathieu Vinken Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Belgium
Vera Rogiers Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Belgium
Editors Mathieu Vinken Department of In Vitro Toxicology and Dermato-Cosmetology Vrije Universiteit Brussel Belgium
Vera Rogiers Department of In Vitro Toxicology and Dermato-Cosmetology Vrije Universiteit Brussel Belgium
ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-2073-0 ISBN 978-1-4939-2074-7 (eBook) DOI 10.1007/ 978-1-4939-2074-7 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014954297 © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any lega