METHODS IN ENZYMOLOGY Editors-in-Chief
JOHN N. ABELSON and MELVIN I. SIMON Division of Biology California Institute of Technology Pasadena, California Founding Editors
SIDNEY P. COLOWICK and NATHAN O. KAPLAN
Academic Press is an imprint of Elsevier 525 B Street, Suite 1900, San Diego, CA 92101-4495, USA 225 Wyman Street, Waltham, MA 02451, USA The Boulevard, Langford Lane, Kidlington, Oxford, OX51GB, UK 32, Jamestown Road, London NW1 7BY, UK Radarweg 29, PO Box 211, 1000 AE Amsterdam, The Netherlands First edition 2013 Copyright © 2013, Elsevier Inc. All Rights Reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, mechanical, photocopying, recording or otherwise without the prior written permission of the publisher Permissions may be sought directly from Elsevier’s Science & Technology Rights Department in Oxford, UK: phone (+44) (0) 1865 843830; fax (+44) (0) 1865 853333; email:
[email protected] Alternatively you can submit your request online by visiting the Elsevier web site at http://elsevier.com/locate/permissions, and selecting Obtaining permission to use Elsevier material Notice No responsibility is assumed by the publisher for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. Because of rapid advances in the medical sciences, in particular, independent verification of diagnoses and drug dosages should be made For information on all Academic Press publications visit our website at store.elsevier.com ISBN: 978-0-12-391861-1 ISSN: 0076-6879 Printed and bound in United States of America 13 14 15 16 11 10 9 8 7 6 5
4 3
2 1
CONTRIBUTORS Herve´ Abdi School of Behavioral and Brain Sciences, The University of Texas at Dallas, Richardson, Texas, USA Ravinder Abrol Materials and Process Simulation Center, California Institute of Technology, Pasadena, California, USA Yashwanth Ashok Oulu Biocenter and Department of Biochemistry, University of Oulu, Oulu, and National Doctoral Programme in Informational and Structural Biology, A˚bo Akademi University, Turku, Finland Jean-Michel Be´cu UMR CNRS 6214—INSERM 1083, Faculte´ de Me´decine, Angers, France Carville G. Bevans Im Hermeshain 6, Frankfurt am Main, Germany Jenelle K. Bray Department of Structural Biology, Stanford Medical School, Stanford, California, USA Florian Brueckner Laboratory of Biomolecular Research, Paul Scherrer Institut, Villigen PSI, Switzerland Gianluigi Caltabiano Laboratori de Medicina Computacional, Unitat de Bioestadı´stica, Facultat de Medicina, Universitat Auto`noma de Barcelona, Barcelona, Spain Mercedes Campillo Laboratori de Medicina Computacional, Unitat de Bioestadı´stica, Facultat de Medicina, Universitat Auto`noma de Barcelona, Barcelona, Spain Pengxiu Cao Department of