Natural Products Isolation
METHODS
IN
BIOTECHNOLOGY”
John M. Walker,SERIES EDITOR 7. Affinity Biosensors:TechniquesandProtocols,editedby Kim R. Rogers and Ashok Mulchandanl, 1998
6. Enzymeand Microbial Biosensors:Techniquesand Protocols,editedby Ashok Mulchandani and Kim R. Rogers, 1998 5. Biopesticides:Use andDelivery,editedby Franklin R. Hall and Julius J. Menn, 1998 4. Natural ProductsIsolation,editedby Richard J. I? Cannell, 1998
3. RecombinantProteinsfrom Plants:ProductionandIsolation of Clinically Useful Compounds,editedby Charles Cunningham and AndrewJ. R Portev, 1998 2. Protocolsin Bioremediation,editedby David Sheehan, 1997 1. Immobilizationof Enzymesand Cells,edltedby Gordon F: Bickerstafi 1997
Natural Products Isolation Edited by
Richard
J. P. Cannell
Glaxo Wellcome Research & Development,
Humana Press
Stevenage, Herb, UK
Totowa, New Jersey
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Library of Congress Cataloging m Pubhcatton Data Mam entry under title Methods m molecular btologyTM Natural products tsolatton/edlted by Richard J P Cannell. p. cm -(Methods m brotechnology, 4) Includes index ISBN o-89603-362-7 (alk paper) I. Natural products 2 Extraction (Chemtstry) I Cannell, Richard J P btotechnology (Totowa, NJ), 4 QD415 N355 1998 547 7-dc21
II Series Methods m 98-16651 CIP
Preface Biodiversity 1sa term commonly used to denote the variety of species and the multiplicity of forms of life. But this variety is deeper than is generally imagined. In addition to the processesof primary metabolism that involve essentially the same chemistry across great swathes of life, there are a myriad of secondary metabolites-natural products-usually confined to a particular group of organisms, or to a single spec