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This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered.
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Copyright © 1994 by ACADEMIC PRESS. INC. All Rights Reserved. No pan of this publkation may be reproduced or transmitted in any form or by any means. electronic or mechanical. including photocopy. recording. or any information storage and retrieval system. without permission in writing from the publisher.
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Table of Contents
CONTRI8UTORS TO VOLUME 237 .
PRt:f"CE
.
VOLUMES IN SERIES
)1'111
Section I. Ga Subunits I.
Measurement of Receptor-Stimul"ted Guanosine THOM"S Wlt:I.AND AND Y-O'(y,ThiO)triphosphate Binding by G Pro- K A RL H. JAKOHS teins
2. Receptor-Slimulated Hydrolysis of Guanosine 5' PETER GIERSCIUK. Triphosphate in Membra.ne PreparJ.tions
THOMAS BOUILLON. AND
K"II.L H. JAKOHS 3. Regulation of G-Protein Activation by Mastopar-lOs and Other Clilionic Pcptides
13
EI.I.IOTT M. Ross AN\} 26
TSUTOMU HIG"SHIJIM"
4. Guanosine 5'-O-(y·Thio)triphosphate Binding As- DoNNA 1. CARTY "NO say for Solubilized G PrOleins
J8
R"vt IYENGAR
5. Activation ofCholem TO)lin by ADP-Ribosylation JOEL M oss
.
Factors: 20-kDa Guanine Nucleotide.Binding R"NOY S. H"UN. Proteins
SU CI IEN TSA1, ,
CATHEII.INE F. Wnsli. FANG-JEN SCOTT LEE,
S. Russ PRICE. AND
6. Pertussis Toxin-Catalyzed ADP·Ribosylation of 0
MARTHA VAUGHAN
44
DoNNA 1. CARTY
63
Proteins 7. Synthesis and Use of Radioactive Pholoaclivat-
R1CIIARD R. VAILLANCOURT.
able NAO' Derivatives as Probes forG-Protein N. DIIANASEKARAN. AND Structure
ARNOLD E. Ruouo
8, PholoaffinilY Guanosine Y-Triphosphate Analogs MARK M. RAS EN1CK
70 .
as a Tool for the Study ofGTP-Binding Proteins MADIIAVI TAI.I.UIU. AND WILLIAM J. DUNN III 9. Prepar.l.tion of Activated a Subunits ofG, andG,s:
From Erythrocyte to Activated Subunit
100
LUTZ BIRNHAUMER, D"GOHERTO GRENET. FERNANDO RI8EIRO-NETO. AND JUAN CODINA
110
TABLE OF COI"'TE",TS
v,
10. l>Urification and Separation of Closely Related TOSHI""I KAT"!),,, Membe� of Pertussis Toxin-Substrate G Pro·
KENJI KONTANI.
teins
ATSUSHI INANORF-. [CHIRQ KOBAYASHI. YOSHIIIARU OIlOK". HIItOSIil NISltlNA. AND KATSUNOHU TAKAHASHI
II. Purification of Tmnsducin
131
JOf!:LLE BIGA\' AND MARC (HARRE
12. Expression orO-Protein n Subunits in £S{'/lI'ridri"
'39
ETUAN LEE. MAURINE E. LINDI,It, AND
coli
Al.FRED G. GII.MAN
'4{\
13. Synthesis and Applications of Affinity Matrix IOK-Hou PAf"G. Containing Immobilized (ly Subunits orG Pro- ALAN V. SM lleK" ,
14. Purification
"I'll)
PAUL C. STERNWEIS
teins of
Activated
and
1