Peptidases And Neuropeptide Processing

Methods in Neurosciences Volume 23 Peptidases and Neuropeptide Processing Methods in Neurosciences Editor-in-Chief P. Michael Conn Methods in Neurosciences Volume 23 Peptidases and Neuropeptide Processing Edited by A. Ian Smith Peptide Biology Laboratory Baker Medical Research Institute Prahran, Victoria Australia ACADEMIC PRESS San Diego New York Boston London Sydney Tokyo Toronto This book is printed on acid-free paper. Copyright 9 1995 by ACADEMIC PRESS, INC. All Rights Reserved. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Academic Press, Inc. A Division of Harcourt Brace & Company 525 B Street, Suite 1900, San Diego, California 92101-4495 United Kingdom Edition published by Academic Press Limited 24-28 Oval Road, London NW1 7DX International Standard Serial Number: 1043-9471 International Standard Book Number: 0-12-185293-8 PRINTED IN THE UNrIED STATES OF AMERICA 95 96 97 98 99 00 EB 9 8 7 6 5 4 3 2 1 Table of Contents Contributors to Volume 23 Preface Volumes in Series ix ~ 1 7 6 1 7 6 Xlll XV Section I Molecular Approaches for the Study of Intracellular Processing Enzymes 1. Molecular Strategies for Identifying Processing Enzymes Nabil G. Seidah 2. In Situ Hybridization Techniques to Map Processing Enzymes 16 Martin K.-H. Schiifer and Robert Day 3. Analysis of Ontogeny of Processing Enzyme Gene Expression and Regulation 45 Min Zheng and John E. Pintar 4. Use of Vaccinia Virus Vectors to Study Neuropeptide Processing 65 Judy K. VanSlyke, Laurel Thomas, and Gary Thomas 5. Overexpression of Neuropeptide Precursors and Processing Enzymes 94 Iris Lindberg and Yi Zhou 6. Use of Antisense RNA to Block Peptide-Processing Enzyme Expression Richard E. Mains 109 Section II Immunological and Biochemical Approaches to the Study of Peptide-Processing Pathways 7. Combination of High-Performance Liquid Chromatography and Radioimmunoassay for Characterization of Peptide-Processing Pathways A. Ian Smith and Rebecca A. Lew 125 8. Development and Use of Two-Site Immunometric Assays for Examining Peptide-Processing Pathways Steven R. Crosby 140 vi TABLE OF CONTENTS 9. Methods for Identification of Neuropeptide-Processing Pathways Paul Cohen, Mohamed Rholam, and Hamadi Boussetta 155 10. Immunological and Related Techniques for Studying Neurohypophyseal Peptide-Processing Pathways Harold Gainer, Mark O. Lively, and Mariana Morris 195 11