E-Book Overview
Written for both novices and experts, this handy paperback describes modern tissue culture methods within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology. The book's unique contribution is its rendering of technical cell culture information in a comprehensive, logical format. Troubleshooting problems are approached from a theoretical perspective, helping the reader to understand and surpass the examples given, while exercises and techniques stimulate thought about the broader aspects of cell culture. Nearly 100 illustrations, including 41 halftones, highlight the book. Appendices include references to relevant Internet sites and other useful sources of information.
E-Book Content
Introduction to Cell and Tissue Culture Theory and Technique huangzhiman 2002.12.18 Foreword Chapter 1: Introduction Chapter 2: Setting Up a Cell Culture Laboratory Chapter 3: The Physical Environment 1 9 25 Chapter 4: Media 41 Chapter 5: Standard Cell Culture Techniques 63 Chapter 6: Looking at Cells 89 Chapter 7: Contamination: How to Avoid It, Recognize It, and Get Rid of It Chapter 8: Serum-Free Culture Chapter 9: Primary Cultures Chapter 10: Establishing a Cell Line Chapter 11: Special Growth Conditions Chapter 12: Cell Culture for Commercial Settings Glossary Appendix Index 117 129 151 165 175 195 205 Page i Introduction to Cell and Tissue Culture Theory and Technique Page ii INTRODUCTORY CELL AND MOLECULAR BIOLOGY TECHNIQUES SERIES EDITOR: Bonnie S. Dunbar, Baylor College of Medicine, Houston, Texas INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P. Mather and Penelope E. Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P. Mather and Penelope