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"...very informative and comprehensive...belongs on the shelf of every library and lab providing essential resources for research on vector design or delivery.-Cancer Biology and TherapyThis comprehensive volume presents the most recent advances in target definition technology and provides a detailed overview on the rational design of targeted vectors for gene therapy. A theoretical framework for advanced vector design is provided that integrates all of the allied sciences relevant to the study of vector targeting. The text discusses the basic underlying science and then leads to discussions of the various viral vectors and methods of defining targets. Finally, an expert outlook on promising therapeutic applications is offered.
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Made by Cellculture 23 May 2003 www.dnathink.org www.biolover.com www.bioin.org Color Figures A B 1745 N 3403 P/V/C 4872 7248 M F 15894 9209 H L Figure 15.1 Schematic representation of (A) the MV genome and (B) an MV particle. The color code for proteins is indicated in the genome scheme. The lipid bilayer surrounding the particle is shown in blue. SacII T7 p(+)MV-NSe p(+)MV-H/EGF p(+)MV-H/IGF p(+)MV-H/αCEA N P NarI M PacI F SpeI H H H H L δ φ EGF IGF αCEA Figure 15.3 Genomic vectors for recovery of recombinant viruses. Plasmid p(+)MVNSe specifies MV antigenomic RNA. Transcription with T7 RNA polymerase from a T7 promoter fused directly to the antigenome sequence yields RNA bearing the authentic nucleotides of the viral antigenomic 5′ terminus. The correct MV 3′ terminus is cleaved precisely by a hepatitis delta virus genomic ribozyme ( ) located immediately adjacent to the MV 3′-terminal