E-Book Overview
The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideo type, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow’s crop varieties.
E-Book Content
Methods in Molecular Biology 1145 Delphine Fleury Ryan Whitford Editors Crop Breeding Methods and Protocols METHODS IN M O L E C U L A R B I O LO G Y Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Crop Breeding Methods and Protocols Edited by Delphine Fleury and Ryan Whitford Australian Centre for Plant Functional Genomics (ACPFG), University of Adelaide, Urrbrae, SA, Australia Editors Delphine Fleury Australian Centre for Plant Functional Genomics (ACPFG) University of Adelaide Urrbrae, SA, Australia Ryan Whitford Australian Centre for Plant Functional Genomics (ACPFG) University of Adelaide Urrbrae, SA, Australia Additional material to this book can be downloaded from http://extras.springer.com ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-0445-7 ISBN 978-1-4939-0446-4 (eBook) DOI 10.1007/978-1-4939-0446-4 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014936846 © Springer Science+Business Media New York 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that su