Xenotransplantation could have an impact on at least three aspects of medicine. The first is as a means of overcoming a severe shortage of human donor organs for the treatment of organ failure. The second aspect relates to the possibility that a xenogeneic organ would not be susceptible to infection by a "human" virus and thus the xenograft might resist injury caused by such viruses. The third and, as of yet, unexplored aspect relates to a means of delivering genes for therapeutic purposes thus overcoming some of the limitations of "conventional" gene therapy.
278 Current Topics in Microbiology and Immunology Editors R.W. Compans, Atlanta/Georgia M.D. Cooper, Birmingham/Alabama · Y. Ito, Singapore H. Koprowski, Philadelphia/Pennsylvania F. Melchers, Basel · M.B.A. Oldstone, La Jolla/California S. Olsnes, Oslo · M. Potter, Bethesda/Maryland P.K. Vogt, La Jolla/California · H. Wagner, Munich Springer-Verlag Berlin Heidelberg GmbH D.R. Salomon · C. Wilson (Eds.) Xenotransplantation With 22 Figures and 17 Tables BD Daniel R. Salomon, M.D. The Scripps Research Institute Department of Molecular and Experimental Medicine MEM 55, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA e-mail:
[email protected] Carolyn Wilson, M.D. U.S. Food and Drug Administration Division of Cellular and Gene Therapies Center for Biologics Evaluation and Research, FDA Building 29B, Room 2NN11 8800 Rockville Pike, HFM-530, Bethesda, MD 20892, USA e-mail:
[email protected] Cover Illustration by C. Wilson: Shown on the front cover is a fluorescent confocal micrograph (630x) of HEK 293 cells productively infected with PERV-A (strain 14/220, provided by Clive Patience, Immerge Therapeutics). Cells were immunostained as follows: 1) Green = Goat antisimian sarcoma-associated virus gp70 (which cross-reacts with PERV gp70 envelope) followed by a FITC donkey-anti-goat anti-serum (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA); 2) Red = Mouse monoclonal anti-human golgin-97 (Molecular Probes, Eugene, OR) followed by a Texas Red donkey-anti-mouse antiserum (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA); and 3) Blue = Topro-3, a fluorescent stain for the cell nucleus (Molecular Probes, Eugene, OR). ISSN 0070-217X ISBN 978-3-642-62431-5 ISBN 978-3-642-55541-1 (eBook) DOI 10.1007/978-3-642-55541-1 Library of Congress Catalog Card Number 72-152360 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. http://www.springer.de Springer-Verlag Berlin Heidelberg 2003 Originally published by Springer-Verlag Berlin Heidelberg New York in 2003 Softcover reprint of the hardcover 1st edition 2003 The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Typesetting: St rtz AG, W rzburg Cover Design: Design & Production GmbH, Heidelberg Production Editor: Ang lique Gcouta, Berlin Prin