The Chlamydomonas Sourcebook: Introduction To Chlamydomonas And Its Laboratory Use, Volume 1, Second Edition

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Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989 has been a classic in the field, and considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for chlamydomonas research and best practices for applications in research including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. * Expanded revision of gold standard reference * Includes latest advances in research including completion of the genome * Provides broad perspective with for studies in cell and molecular biology, genetics, plant physiology and related fields

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The Chlamydomonas Sourcebook SECOND EDITION Volume 1: Introduction to Chlamydomonas and Its Laboratory Use i This page intentionally left blank The Chlamydomonas Sourcebook SECOND EDITION Volume 1: Introduction to Chlamydomonas and Its Laboratory Use Elizabeth H. Harris, Ph.D. Department of Biology Duke University Durham, North Carolina, USA AMSTERDAM • BOSTON • HEIDELBERG • LONDON • NEW YORK OXFORD • PARIS • SAN DIEGO • SAN FRANCISCO • SYDNEY • TOKYO Academic Press is an imprint of Elsevier Cover image: A cluster of post-mitotic Chlamydomonas daughter cells from a mother cell that underwent two rounds of division. Courtesy of Su-chiung Fang and James Umen. Academic Press is an imprint of Elsevier The Boulevard, Langford Lane, Kidlington, Oxford OX5 8GB 30 Corporate Drive, Suite 400, Burlington, MA 01803, USA 525 B Street, Suite 1900, San Diego, CA 92101-4495, USA First edition 1989 Second edition 2009 Copyright 2009 © Elsevier Inc. All rights reserved No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, mechanical, photocopying, recording or otherwise without the prior written permission of the publisher. Permissions may be sought directly from Elsevier’s Science & Technology Rights Department in Oxford, UK: phone ( 44) (0) 1865 843830; fax ( 44) (0) 1865 853333; email: [email protected] Alternatively you can submit your request online by visiting the Elsevier web site at http://elsevier.com/locate/permissions, and selecting Obtaining permission to use Elsevier material. Notice No responsibility is assumed by the publisher for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. Because of rapid advances in the medical sciences, in particular, independent verification of diagnoses and drug dosages should be made. Set ISBN: 978-0-12-370873-1 Volume 1: ISBN: 978-0-12-370874-8 Volume 2: ISBN: 978-0-12-370875-5 Volume 3: ISBN: 978-0-12-370876-2 For information on all Academic Press publications visit our website at www.elsevierdirect.com Typeset by Charon Tec Ltd., A Macmillan Company. (www.macmillansolutions.com) Printed and bound in Canada 09 10 11 12 10 9 8 7 6 5 4 3 2 1 Contents of Volume 1 Preface ................................................................................................